Butterworth Lab Department of Cell Biology University of Pittsburgh
Butterworth LabDepartment of Cell BiologyUniversity of Pittsburgh


42) Farrell C.E., Liu X., Yagan N.O., Suda A.C., Cerqueira D.M., Bodnar A.J., Kashlan O.B., Subramanya A.R., Ho J., Butterworth M.B. (2023) MicroRNA-19 is regulated by aldosterone in a sex-specific manner to alter kidney sodium transport. Am J Physiol Cell Physiol. In Press.

 PMID: 3804729


41)  Kolski-Andreaco A, Balut CM, Bertuccio CA, Wilson AS, Rivers WM, Liu X, Gandley RE, Straub AC, Butterworth MB, Binion DG, Devor DC. (2022) Histone Deacetylase Inhibitors (HDACi) Increase Expression of KCa2.3 (SK3) in Primary Microvascular Endothelial Cells.

Am J Physiol Cell Physiol. 322(3):C338-C353



40) Butterworth, M.B. (2021). Non-coding RNAs and the mineralocorticoid receptor in the kidney. Mol. Cell Endocrinol. 521:111115. 

PMID: 33301840 


39) Ozbaki-Yagan, N.,  Liu, X., Bodnar, A.J., Ho, J. and Butterworth, M.B. (2020). Aldosterone-induced microRNAs act as feedback regulators of mineralocorticoid receptor signaling in kidney epithelia. FASEB J. 34(9):11714-11728.

PMID: 32652691


38) Phua YL, Chen KH, Hemker SL, Marrone AK, Bodnar AJ, Liu X, Clugston A, Kostka D, Butterworth MB, Ho J. (2019). Loss of miR-17~92 results in dysregulation of Cftr in nephron progenitors. American Journal of Physiology - Renal Physiology. 316(5):F993-F1005.

PMID: 30838872


37) Butterworth MB, Alvarez de la Rosa D. (2019). Regulation of Aldosterone Signaling by MicroRNAs. Vitam Horm. 109:69-103

PMID: 30678867


36) Espiritu E.B., Crunk A.E., Bais A., Hochbaum D., Cervino A.S., Phua Y.L., Butterworth M.B., Goto T., Ho J., Hukriede N.A., Cirio M.C. (2018). The Lhx1-Ldb1 complex interacts with Furry to regulate microRNA expression during pronephric kidney development. Scientific Reports. 8(1):16029. PMID: 30375416


35) Butterworth MB. (2018). Role of microRNAs in aldosterone signaling. Current Opinion in Nephrology and Hypertension. 27(5):390-394.

PMID: 30074910


34) Butterworth MB.(2018). The Tale of Two (Distal Nephron) Cell Types.  Am J Physiol Renal Physiol. 314 (5):F930-F931.
PMID: 29363323


33) Mukherjee, A., Wang, Z., Kinlough, C.L., Poland, P.A., Marciszyn, A.L., Montalbetti, N., Carattino, M.D., Butterworth, M.B., Kleyman, T.R. and Hughey, R.P. (2017). Specific palmitoyltransferases associate with and activate the Epithelial Sodium Channel. Journal of Biological Chemistry. 292(10):4152-4163



32) Klemens, C.A, Edinger, R.S, Kightlinger, L., Liu, X. and Butterworth, M.B. (2017). Ankyrin G Expression Regulates Apical Delivery of the Epithelial Sodium Channel (ENaC). J. Biol. Chem. 292(1):375-385.

PMID: 27895120


31) Liu X, Edinger RS, Klemens CA, Phua YL, Bodnar AJ, LaFramboise WA, Ho J, Butterworth MB. (2016). A MicroRNA Cluster miR-232427 Is Upregulated by Aldosterone in the Distal Kidney Nephron where It Alters Sodium Transport. J Cell Physiol. 232(6):1306-1317.

PMID: 27636893


30) Li Y, Hu H, Butterworth MB, Tian JB, Zhu MX, O'Neil RG. (2016). Expression of a Diverse Array of Ca2+-Activated K+ Channels (SK1/3, IK1, BK) that Functionally Couple to the Mechanosensitive TRPV4 Channel in the Collecting Duct System of Kidney. PLoS One. 11(5):e0155006

PMID: 27159616


29) Butterworth, M.B. (2015). MicroRNAs and the regulation of aldosterone signaling. American Journal of Physiology-Cell Physiol.  308(7):C521-C527.


28) Roy, A., Al-Qusairi, L., Donnelly, B.F., Ronzaud, C., Marciszyn, A., Gong, F., Chang, Y.P, Butterworth, M.B., Pastor-Soler, N., Hallows, K.R., Staub, O. and Subramanya, A.R. (2015). Alternatively spliced proline-rich cassettes link WNK1 to aldosterone-dependent signaling cascades.  Journal of Clinical Investigation. 125(9):3433-48.

PMID: 26241057


27) Butterworth, M.B., Zhang, L and Thibodeau, P.H. (2014). Modulation of the Proteolytic Activation of the Epithelial Sodium Channel (ENaC) by a Pseudomonas aeruginosa Protease Inhibitor. PLOS One. 9(6): e100313.

PMID: 24963801


26) Edinger. R.S., Coronnello. C., Bodnar, A.J., LaFramboise, W.A., Benos, P.V., Ho, J., Johnson, J.P and Butterworth, M.B. (2014). Aldosterone regulates microRNAs in the CCD to alter sodium transport. Journal of the American Society of Nephrology. 25 (11):2445-57.


25) Bertuccio, C.A., Lee, S-L., Wu, G., Butterworth, M.B., Hamilton, K.L. and Devor, D.C. (2014). Anterograde trafficking of KCa3.1 in polarized epithelia is Rab1- and Rab8-dependent and recycling endosome-independent. PLOS One. 9(3):e92013

24) Thibodeau P.H. and Butterworth M.B. (2012). Proteases, Cystic Fibrosis and the Epithelial Sodium Channel (ENaC). Cell & Tissue Research 351(2):309-23


23) Edinger, R.S., Bertrand, C.A, Rondandino,C., Apodaca,G.A., Johnson, J.P. and Butterworth, M.B. (2012). The epithelial sodium channel (ENaC) establishes a trafficking vesicle pool responsible for its regulation. PLOS One 7 (9):e46593.


22) Butterworth, M.B., Zhang L., Heidrich E.M., Myerburg M.M., Thibodeau P.H. (2012). Activation of the epithelial sodium channel (ENaC) by the alkaline protease from Pseudomonas aeruginosa. Journal of Biological Chemistry. 287(39):32556-65.


21) Butterworth M.B., Edinger R.S., Silvis M.R., Gallo L.I., Liang X., Apodaca G., Frizzell R.A., Johnson J.P. (2012). RAB11b REGULATES THE TRAFFICKING AND RECYCLING OF THE EPITHELIAL SODIUM CHANNEL (ENaC). Am. J. Physiol. Renal Physiol. 302:F581-90.


20) Myerburg M.M., Harvey P.R., Heidrich E.M., Pilewski J.M., Butterworth M.B. (2010). Acute regulation of ENaC in airway epithelia by proteases and trafficking. American Journal of Respiratory Cell and Molecular Biology. 43(6): 712-9.


19) Butterworth, M.B. (2010). Regulation of the epithelial sodium channel (ENaC) activity by membrane trafficking. Biochim Biophys Acta – Molecular Basis for Disease. 1802 (12): 1166-77


18) Liang, X.; Peters, K.W.; Butterworth, M.B., Frizzell, R.A. (2010). AS160 modulates aldosterone stimulated epithelial sodium channel (ENaC) forward trafficking. Molecular Biology of the Cell. 21(12):2024-2033.


17) Hallows, K.R.; Edinger, R.S.; Butterworth, M.B.; Oyster, N.M.; Li, H.; Wang, H.; Buck, J.; Levin, L.R.; Johnson, J.P. and Pastor-Soler, N.M. (2009). Novel regulation of epithelial Na+ transport by soluble adenylyl cyclase in kidney collecting duct cells. Journal of Biological Chemistry. 284(9): 5774-83


16) Butterworth, M.B.; Edinger, R.S.; Frizzell, R.A. and Johnson, J.P.  (2009).  Regulation of the epithelial sodium channel (ENaC) by membrane trafficking.  American Journal of Physiology – Renal.  296: F10-F24


15) Silvis, M.R.; Bertrand, C.A.; Ameen, N.; Golin-Bisello, F.; Butterworth, M.B., Frizzell, R.A. and Bradbury, N.A. (2009).  Rab11b regulates the apical recycling of CFTR in polarized intestinal epithelial cells. Molecular Biology of the Cell. 20(8): 2337-50


14) Butterworth, M.B; Weisz, O.A. and Johnson, J.P.  (2008).  Some assembly required:  Putting the epithelial sodium channel together.  Journal of Biological Chemistry.  283(51): 35305-9


13) Liang, X.; Butterworth, M.B. ; Peters, K.W., Walker, W.H. and Frizzell, R.A. (2008). An obligatory heterodimer of 14-3-3 beta and 14-3-3 epsilon is required for aldosterone regulation of the epithelial sodium channel. Journal of Biological Chemistry. 283: 27418-27425


12) Butterworth, M.B. and Johnson, J.P  (2008).  USP10 the Nexus between Nexin and Vasopressin. American Journal of Pysiology – Renal.  295(4): F888


11) Butterworth, M.B.; Edinger, R.S.; Ovaa, H.; Johnson, J.P. and Frizzell, R.A.  (2007). The deubiquitinating enzyme UCH-L3 regulates the apical membrane recycling of the epithelial sodium channel.  Journal of Biological Chemistry. 282:37885-93


10) Hill, W.G.; Butterworth, M.B*.; Wang, H. Edinger, R.S.; Frizzell, R.A. and Johnson, J.P. (2007).  Lipid rafts mediate constitutive apical delivery of the epithelial sodium channel (ENaC).  Journal of Biological Chemistry. 282:37402-11 (*Co-first author)


9) Myerburg, M.M.; Butterworth, M.B.; McKenna, E.; Frizzell, R.A.; Kleyman,T.R. and Pilewski, J.M.(2006).  Airway surface liquid depth regulates ENaC by altering the serine protease-protease inhibitor balance: A mechanism for sodium hyperabsorption in cystic fibrosis. Journal of Biological Chemistry. 281: 27942-49.


8) Liang, X.; Peters, K.W.; Butterworth, M.B. and Frizzell, R.A. (2006). 14-3-3 isoforms are induced by aldosterone and participate in its regulation of epithelial sodium channels. Journal of Biological Chemistry. 281: 16323-32.


7) Wang, H.; Traub, L.; Weixel, K.; Shah, N.; Edinger, R.S.; Perry C.; Kleyman T.R.; Butterworth M.B.; Frizzell, R.A. and  Johnson, J.P.  (2006).  Clathrin-associated endocytosis of ENaC: role of epsin. Journal of Biological Chemistry. 281: 14129-35.


6) Zhang, H. ; Schmidt, B.Z.; Sun, F.; Condliffe, S.B.; Butterworth, M.B.; Youker, R.T.; Brodsky, J.L.; Aridor, M and Raymond A. Frizzell (2006).  Cysteine string protein monitors late steps in CFTR biogenesis.  Journal of Biological Chemistry.  281: 11312-21.


5) Butterworth, M.B.; Frizzell, R.A.; Johnson, J.P; Peters, K.W. and Edinger, R.S.  (2005).  PKA-dependant ENaC trafficking requires the SNARE binding protein complexin.  American Journal of Physiology-Renal. 285(5):F969-977.


4) Butterworth, M.B.; Edinger, R.S.; Johnson, J.P and Frizzell, R.A.  (2005). Acute ENaC regulation by cAMP in a kidney cell line is mediated by exocytic insertion from a recycling channel pool.  Journal of General Physiology. 125: 81-101.


3) Blazer-Yost, B.L.; Butterworth, M.; Hartman, A.; Parker, G.E.; Faletti, C.; Els, W.J. and Rhodes, S.J. (2001). Characterization of A6 cell clones expressing GFP-labeled ENaC subunits.  American Journal of Physiology-Cell Physiol.  281(2):C624-32.


2) Butterworth, M.B.; Helman, S.I. and Els, W.J.(2000).  cAMP-sensitive endocytic trafficking in A6 epithelia. American Journal of Physiology-Cell Physiol.  280(4): C752-C762.


1) Els, W.J. and Butterworth, M.B. (1998).  The localisation of adenylate cyclase in membranes of cultured renal cells.  Journal of Microscopy Research & Technique 40:455-462.

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Michael Butterworth, PhD